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Here are some more information for Chromatography Column:

Numerous Uses of Acids

Acids are referred to sulfuric, acetic, electrolyte, nitric, carboxylic, monoprotic, phosphoric, etc

Acids are ionic compounds with a positive or negative charge. Generally the taste of acids is sour and it can be in any form gases, liquids and solids. Acids strongly react with metals and other good conductor of electricity. Some corrosive type of acids are strong in acidic power, which are dangerous or unsafe, it may cause burn in just physical touch.

Acids are normally use in industries, household cleaning product, daily products, soft drinks. We can get acidic or sour taste from most of food items such as lemons, sodas, vinegar, starburst, cream, yogurt, cottage cheese, etc. Acids are also widely use in car battery sulfuric acid, wet cell battery electrolyte. Phosphoric acid normally used in cola soft drinks.

Everyday, almost people around the world used these acids as direct or indirect substances. Most chemical industries, laboratories, pharmaceuticals, school labs, college labs and industrial labs required acids. Some of companies from India are best in manufacturing chemicals which contain acids. All manufactures, suppliers, dealers, distributors, importers and exporters of Lab Chemicals, Laboratory Products, Pharma Raw Materials, Scientific Instruments, Bio-chemicals, Medical Equipments, Glassware, Liquid Handling and other allied companies are dependent on chemical needs like “Acids”.

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effect on the results of a column chromatography?

what would be the effect on the results of a column chromatography if metanol were not completely removed from the leaf extract before chromatography

I wonder if this question belongs more in the Chemistry section - but then again chromatography is used in the life sciencies pretty much.

Remember that chromatography separates a mixture into its components. However, oftentimes, a very complex mixture needs to be "cleaned up" by other means prior to the actual chromatography is carried out. Clean up can be via several means, including liquid / liquid extraction (perhaps enhanced via pH adjustment), precipitation, or other means depending upon the specific mix you have.

If the clean up does not occur, the mix may not separate.

Remember that the separation occurs due to polarity difference among elements in the mix in most column chromatography processes. The stationary phase (often a silica) is very polar; the elution solvent is far less polar and as solvent passes over the column, a continual absorption onto the stationary phase followed by dissolution into the liquid phase occurs. The separation happens because some components have a higher affinity for solvent than others.

If a component that is not cleaned up beforehand is added to the column, it can greatly interfere with the absorption / desorption process that enables the separation.

This is what makes the separation complex especially when you are developing an analytical method in a complex matrix.

For your specific example, I suspect the clean up removes components that would foul the separation.

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