Best Lab Equipment

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Discover Details About Utilizing A Microscope In Koi Treatment

A microscope is an important piece of equipment, because it can help you to diagnose parasitic infestation accurately. Microscopes can be highly sophisticated devices. Some offer three dimensional devices; others allow you to take photographs. While these features may be desirable in some circumstances, a simple monocular microscope with fine focus control will normally suffice. An adjustable stage and lighting unit make it easier to observe the slides.

Set up the microscope before taking a scrape. Viewing the scrape shortly after taking it will make the movement of the still live parasites on the slide easier to spot. To look for parasites, use a wooden spatula to take a number of scrapes from a koi suspected of harboring them. The three areas from which to take scrapes are normally just behind the operculum, alongside the dorsal fin and in the caudal region.

Moving from head to tail, draw the side edge of the spatula along the body in line with the scales. This action scrapes some mucus from the scales. Spread the mucus over the central portion of the slide, apply a drop of pond water and press a cover glass into place. Place the slide on the microscope stage and scan it until you see the parasites. Start scanning at a low magnification so that you can view a larger area of the specimen.

When you locate a parasite, switch to a more powerful lens to make a positive identification. A combination of eyepieces and objective lenses to give a range of 50 to 500 times magnification will be sufficient. For best results, you may need to adjust the light level and condenser.

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How do you use an oil immersion microscope; and what kind of "oil" is used and where do you get it?

Trying to teach myself how to use a binocular and monocular microscope for a school research project.

oil immersion is usually used for the highest power of the microscope. It should only be used for microscopes designed for oil immersion.

You would first focus properly at a lower power then when you are ready to use the highest power, put a drop or two of the oil on the slide cover and switch the objective lens. At this point you would only adjust the focus using the fine adjustment knob.

When you are done, wipe down the objective lens with lens paper to remove excessive oil. If you are done with the microscope, use a small amount lens cleaner also to remove most of the oil. No need to completely clean off the objective lens if you are planning on examining additional slides.

Different oils are used for commercially available immersion oils but the one thing they all have in common is the index of refraction (how much they bend light). The index of refraction for immersion oils is always close to the index of refraction for the glass in the slides and cover slips. This maximizes the amount of clarity and contrast of the image by minimizing the effects of light bending from moving through different substances.

If you do not own the microscope, only use commercially available immersion oil (should be available from any place that sells slides). Many microscopes that use oil immersion can cost thousands or tens of thousands of dollars.

If you have the owner's permission or you own the microscope, filtered caster oil has an index of refraction very close to glass and is a suitable substitute.

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