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Spectrophotometry and Its Working Principle

Spectrophotometry is one of the most important practices carried out in physics laboratories. Spectrophotometry is the study of the reflection or transmission properties of a substance as a function of wavelength. It is the quantitative study of electromagnetic spectra of a material. During the process, the transmittance or reflectance of the substance is measured through the careful geometrical and spectral consideration.

To carry out spectrophotometry, a spectrophotometer is required. A spectrophotometer is a device or more specifically a photometer that is used to measure intensity of light as a function of the wavelength of light. Different types of spectrophotometers are found in the market. They are classified on various basis. The most important distinctions used to classify them are:

• The different measurement techniques.
• The wavelengths they work with.
• How they acquire a spectrum.
• The sources of intensity variation.

Principle of Spectrophotometry
A spectrophotometer, one of the most useful physics lab equipments, is the combination of two devices, a spectrometer and a photometer. Spectrometer is used for producing light of any selected wavelength or color while a photometer is used for measuring the intensity of light. The two devices are placed at either side of a cuvette filled with a liquid. Spectrometer produces the light of desired wavelength and it passes through the tube and reaches photometer that measures its intensity. Then the photometer produces a voltage signal to a display device, usually a galvanometer. As the amount of light absorbed by the liquid changes the signal also changes. The concentration of a substance in solution can be measured by calculating the amount of absorption of light at the appropriate wavelength or a particular color.

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photon energy og green lightwaves?

A cheap spectrophotometer can be made using a light-emitting diode as the source of the light. Assume that you build one of these spectrometers using an LED that gives off green light with a wavelength of 520 nm. What is the energy of a photon of this green light?

First, convert the wavelength to frequency via the equation: c= (wavelength) * (frequency), where c is the speed of light (3.0e8 m/s). Then, slip the frequency into the next equation: E= h* (frequency)....E will be the energy in joules, and h is Plank's constant....can't remeber the value off the top of my head, but the units are joule-seconds....

Non-Contact Imaging Color Spectrophotometer Provides 1.2 Million Independent Spectral Reflectance Curves in Single ...
TRICOR Systems, Inc., Elgin IL, designer / manufacturer of the first imaging gloss meter, is proud to introduce another first - The Model 600 Non-Contact Imaging Spectrophotometer.

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