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What You Need to Know About Spectrophotometers

Spectrophotometry is used in physics in studying electromagnetic spectra, specifically dealing with near ultraviolet, near infra-red, and visible light. The process is done with the use of an instrument known as the spectrophotometer, since it can measure the intensity, color, and wavelength of light.

A NIR spectrophotometer is commonly used to measure light absorption and diffuse or specular reflectance. However, spectrophotometers are not only used in physics; it can also be used in other fields such as biochemistry, chemistry, and molecular biology. Any industry that requires forensic examination makes use of a NIR spectrophotometer.

Spectrophotometers come in two designs – single beam and double beam. In the single beam spectrophotometer, the relative light intensity is measured before and after the sample is inserted. Double beam spectrophotometers are used to compare two light paths, the first one being a reference sample and the second one being a test sample. While it does seem that double beam spectrophotometers are more beneficial in terms of comparing light beams, single beam spectrophotometers have the advantage of a larger dynamic range.

A NIR spectrophotometer works by first inserting the sample into the instrument. You must have a light source that shines into the monochromator. A particular wavelength that is beamed at the sample is selected, and in turn, the sample absorbs the light. Behind the sample is a photodetector that responds to the light stimulus and gives you an analog electronic current. The photodetector then converts this current into a usable format. These numbers are then plotted or manipulated via a computer.

There are two common kinds of spectrophotometers: the UV VIS NIR spectrophotometer, and the IR spectrophotometer. The former stands for ultraviolet visible near infrared spectrophotometer. It is so called because the light used is in the visible and adjacent ranges, such as the near ultraviolet and near infrared ranges. It is often used in colorimetry science, such as ink manufacturers, textile vendors, and printing companies, because the color of the chemicals is affected by the absorption of light in the visible ranges.

The second type of NIR spectrophotometer is the IR or infrared spectrophotometer. It is designed for the main infrared region and is less commonly used compared to the UV VIS NIR spectrophotometer.

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can you give me a detailed information bout Atomic absorption spectrophotometer, ICP and UV-VIS? thank you. i badly need this.

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Thermo Fisher Scientific Introduces New Microplate Spectrophotometer
Thermo Fisher Scientific Inc., the world leader in serving science, recently introduced the new Thermo Scientific Multiskan GO UV-Vis spectrophotometer offering free wavelength selection for both 96- and 384-well plates and various types of cuvettes.

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